Donors : 200

Naïve Human scFv Library as phage particles: (~1.0×10¹³ pfu/mL, Ampicillin resistant), supplied in 1000μL PBS with 15% glycerol.

E.coli TG1 host strain: K12 D(lac-pro), supE, thi, hsdD5/F’[traD36, proAB, lacIq, lacZDM15].  

E.coli HB2151 host strain: K12 D(lac-pro), ara, nalr, thi/F’[proAB, lacIq, lacZDM15]. BS1365 host strain: BS591 F’ kan (BS591: recA1 end A1 gyrA96 thi-1 D lacU169 supE44 hsdR17 [lambda imm434 nin5 X1-cre].

M13K07 helper phage: 1.0×10¹² pfu/mL supplied in 100μL PBS with 15% glycerol(Kanamycin resistant).

F primer:  1OD, 10 pmol/μL  

R primer: 1OD, 10 pmol/μL